Figure 4

The Effect of melittin treatment on the post-translation modification of α-synuclein in symptomatic hSOD1 ^G93A mice. The western blot image is representative of three independent experiments. Higher molecular weight forms of α-synuclein were expressed in spinal cord of hSOD1^G93A mice, but this expression was reduced following metlittin treatment (A). The level of nitrated (B) and phosphorylated α-synuclein (C) was decreased in the brainstem and spinal cord of melittin-treated mice. The expression of HSP70 was increased by melittin treatment in the brainstem and spinal cord in hSOD1^G93A mice (D). HSC70 (E) and tubulin (F) were used as loading controls.