General schematic for induction of EAE by adoptive transfer into C57BL/6 mice. Groups of donor mice were immunized with CFA/(MOG)35-55. Some mice also received pertussis toxin (PT) on the day of immunization (day 0) and on day 2 post immunization. Lymph node cells (LNC) and splenocytes SPC) were harvested at either day 6 or day 12 post immunization and were restimulated in vitro with a combination of MOGp35-55 and IL-2, or MOGp35-55 and IL-12 for 3-8 days in tissue culture flasks. After specified days in culture, 10-20 × 106 cells in 200 μL PBS were injected into recipient mice. Mice were observed every day after injection for disease onset and clinically scored.