Flow cytometry analysis of expression of T-bet by encephalitogenic CD4
+ T cells is maintained in Day 12 LNC. C57BL/6 donor mice were immunized with CFA/MOGp35-55 followed by PT at day 0 and day2. At day 6 or day 12, LNC and SPC were harvested and cultured for 72 hours in the presence of MOGp35-55 and IL-12 and prepared for flow cytometry. CD3+CD4+CD44+ T cells were examined for expression of T-bet. The percentage of T-bet+ cell is indicated in the quadrants B, D, F, & H. Isotype controls are shown in panels A, C, E, and G. Data shown are representative of at least 3 independent experiments.