Figure 1
From: Fingolimod modulates microglial activation to augment markers of remyelination
Spheroid myelinating cultures were prepared from fetal rat telencephalon and demyelinated with lysophosphotidyl choline on DIV 25-29 in the presence or absence of fingolimod. Myelin basic protein (A) and neurofilament (B) levels were measured using protein specific ELISA following demyelination. C) Spheroids were fixed and processed for electron microscopy. The mean ratio (+/- SEM) of fibre diameter to myelin thickness was determined (G-ratio) in a minimum of 10 fibres per sample from 3 samples per group. Results were analysed by ANOVA with Holm-Sidak post test. * = p < 0.05; ** = p < 0.01.