Figure 3
From: Fingolimod modulates microglial activation to augment markers of remyelination
Spheroid myelinating cultures were prepared from fetal rat telencephalon and demyelinated with lysophosphotidyl choline on DIV 25-29 in the presence or absence of fingolimod. Samples of spheroids were homogenised in protein extraction buffer, centrifuged and the supernatant removed. Samples were analysed by ELISA for (A) ferritin and (B) nitric oxide over the course of demyelination and remyelination. Results were analysed by ANOVA with Holm-Sidak post test, and are expressed as mean +/- SEM, n = 10. * = p < 0.05; ** = p < 0.01.