Effect of GSNO and SIN-1 on BBB leakage and edema. Photographs showing Evan's blue (EB) extravasations in five coronal sections of brain starting at 4 h after TBI. Animals were sacrificed at 48 h, the brain was sectioned and photographed (A) and the intensity of EB (B) was determined by spectrofluorometric estimation. EB extravasations were not observed in sham brain, hence the photographs of sham brain are not shown. Edema (C) was measured at 24 h after TBI. While GSNO treatment of TBI decreased, SIN-1treatment increased both the Evan's blue extravasations and edema. Data are expressed as mean ± SD from five different experiments. ***p < 0.001 vs. TBI and SIN-1, *p < 0.05 vs. TBI.