Effects of C5a on heat responses of mechanosensitive C-fibers in incised tissue. (A) Example recordings of C-fibers with receptive fields ≤ 2 mm from the incision after application of PBS-vehicle or C5a 1 μg/ml. (B-D, F-H and J-L) Heat responses of C-fibers after exposure to C5a (1 or 0.1 μg/ml) or PBS-vehicle which have receptive fields ≤ 2 mm (F-H) and > 2 mm (J-L) from the incision and in the sham group (B-D). The proportion of C-fibers responsive to heat after application of each drug (E, I and M). Heat thresholds (B, F and J) and total action potentials for 20 s during heat application (C, G and K) and 40 s after heat application (D, H and L). Each symbol represents a single unit. *, P < 0.05. **, P < 0.01 vs PBS-vehicle. Data are presented as median values.