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Table 1 Confirmation of the ERα agonist-regulated immunity/inflammation genes by quantitative real-time PCR.

From: Estrogens regulate neuroinflammatory genes via estrogen receptors α and β in the frontal cortex of middle-aged female rats

GENE MICROARRAY REAL-TIME PCR
Symbol Name probe set FC TaqMan ID RQ
S100a9 S100 calcium binding protein A9 1387125_at 3.655 Rn00585879_m1 2.029
S100a8 S100 calcium binding protein A8 1368494_at 3.458 Rn00587579_g1 2.558
RT1-Aw2 RT1 class Ib 1388202_at 0.291 Rn03034964_u1 0.658
RatNP-3b defensin ratNP-3 precursor 13700791_at 3.317 Rn01478511_gH 10.568
IgG-2a immonoglobulin G 1370394_at 3.204 Rn01429839_g1 9.257
Np4 defensin NP-4 precursor 1370470_at 3.031 Rn00597762_g1 2.268
Defa defensin, alpha 5, Paneth cell-specific 1387943_at 2.732 Rn02607254_g1 32.763
Prg2 proteoglycan 2 1387633_at 2.412 Rn00581137_m1 1.257
Camp cathelicidin antimicrobial peptide 1393603_at 2.297 Rn01446021_g1 1.521
Mcpt9 mast cell protease 9 1368501_s_at 2.235 Rn00755366_g1 5.825
Fcgr2b Fc fragment of IgG, receptor 1371079_at 0.451 Rn00598391_m1 0.649
Igh-1a immunoglobulin heavy chain 1388272_at 2.144   
Cd74 CD74 1367679_at 0.473 Rn00565062_m1 0.685
Fcnb ficolin beta 1387378_at 2.071 Rn00586231_m1 1.922
Mcpt8 mast cell protease 8 1369586_at 2.056 Rn01789238_g1 3.784
Igj immunoglobulin joining chain 1383163_at 1.945 Rn01768305_m1 1.204
Igha immunoglobulin heavy chain, alpha 1371262_at 1.866   
RT1-Aw2 RT1 class Ib 1388203_x_at 0.536 Rn03034964_u1 0.658
Serping1 C1-Inhibitor 1372254_at 0.559 Rn01485600_m1 0.690
C3 complement C3 1368000_at 0.559 Rn00566466_m1 0.743
RT1-N1 RT1 class Ib, locus N1 1387839_at 0.570 Rn00561858_m1 0.654
Lrrc8a leucine rich repeat containing 8 family 1382920_at 1.670   
  1. Transcriptional regulation of twenty-one immunity genes determined by the top100 probe sets of microarray analysis was confirmed by real-time PCR. FC, fold change; RQ, relative quantity.