Estrogens regulate neuroinflammatory genes via estrogen receptors α and β in the frontal cortex of middle-aged female rats

Table 1 Confirmation of the ERα agonist-regulated immunity/inflammation genes by quantitative real-time PCR.

GENE		MICROARRAY		REAL-TIME PCR
Symbol	Name	probe set	FC	TaqMan ID	RQ
*S100a9*	S100 calcium binding protein A9	1387125_at	3.655	Rn00585879_m1	2.029
*S100a8*	S100 calcium binding protein A8	1368494_at	3.458	Rn00587579_g1	2.558
*RT1-Aw2*	RT1 class Ib	1388202_at	0.291	Rn03034964_u1	0.658
*RatNP-3b*	defensin ratNP-3 precursor	13700791_at	3.317	Rn01478511_gH	10.568
*IgG-2a*	immonoglobulin G	1370394_at	3.204	Rn01429839_g1	9.257
*Np4*	defensin NP-4 precursor	1370470_at	3.031	Rn00597762_g1	2.268
*Defa*	defensin, alpha 5, Paneth cell-specific	1387943_at	2.732	Rn02607254_g1	32.763
*Prg2*	proteoglycan 2	1387633_at	2.412	Rn00581137_m1	1.257
*Camp*	cathelicidin antimicrobial peptide	1393603_at	2.297	Rn01446021_g1	1.521
*Mcpt9*	mast cell protease 9	1368501_s_at	2.235	Rn00755366_g1	5.825
*Fcgr2b*	Fc fragment of IgG, receptor	1371079_at	0.451	Rn00598391_m1	0.649
*Igh-1a*	immunoglobulin heavy chain	1388272_at	2.144
*Cd74*	CD74	1367679_at	0.473	Rn00565062_m1	0.685
*Fcnb*	ficolin beta	1387378_at	2.071	Rn00586231_m1	1.922
*Mcpt8*	mast cell protease 8	1369586_at	2.056	Rn01789238_g1	3.784
*Igj*	immunoglobulin joining chain	1383163_at	1.945	Rn01768305_m1	1.204
*Igha*	immunoglobulin heavy chain, alpha	1371262_at	1.866
*RT1-Aw2*	RT1 class Ib	1388203_x_at	0.536	Rn03034964_u1	0.658
*Serping1*	C1-Inhibitor	1372254_at	0.559	Rn01485600_m1	0.690
C3	complement C3	1368000_at	0.559	Rn00566466_m1	0.743
*RT1-N1*	RT1 class Ib, locus N1	1387839_at	0.570	Rn00561858_m1	0.654
*Lrrc8a*	leucine rich repeat containing 8 family	1382920_at	1.670

Transcriptional regulation of twenty-one immunity genes determined by the top100 probe sets of microarray analysis was confirmed by real-time PCR. FC, fold change; RQ, relative quantity.

ISSN: 1742-2094