Figure 5
From: Heteroarylketones inhibit astroglial interleukin-6 expression via a STAT3/NF-κB signaling pathway
Suppression of LPS-induced IL-6 release in primary murine astrocytes by selected HAK compounds. Compounds HAK-2 and HAK-5 significantly reduce LPS-induced IL-6 release in primary mouse (A) and rat astrocytes (B). Astrocytes derived from newborn mice and rats were cultivated for 33 days followed by stimulation with 1 μg/ml LPS and treatment with compounds HAK-2 and HAK-5 (20 μM). After 24 hours conditioned media were analyzed with IL-6 specific ELISA. Data (mean ± SD) were obtained from experiments carried out in quadruplicate and statistical analysis was performed using the paired t test. Amount of IL-6 in LPS-treated control sample was set to 100%. ns, not significant; **p < 0.01; ***p < 0.001.