Effect of compound HAK-2 on OSM-induced STAT3/p65 complex formation in U343 cells. OSM treatment led to increased STAT3/p65 complexation, which is suppressed by compound HAK-2. U343 cells were stimulated with 100 ng/ml OSM and co-treated with compound HAK-2 (20 μM) for 6 h. After treatment, cells were lysed and whole-cell extracts were immunoprecipitated with a polyclonal antibody against the NF-κB subunit p65 or vehicle (control). Immunoblots were prepared and probed with polyclonal antibody against STAT3. Data from a representative experiment (n = 2) are shown.