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Figure 1 | Journal of Neuroinflammation

Figure 1

From: ELISA measurement of specific non-antigen-bound antibodies to Aβ1-42 monomer and soluble oligomers in sera from Alzheimer's disease, mild cognitively impaired, and noncognitively impaired subjects

Figure 1

ELISA plate configuration used to measure specific antibodies to Aβ1-42 monomer and soluble oligomers. Antibodies to Aβ1-42 (both monomer and soluble oligomers) were measured on a separate ELISA plate for each serum sample. The plate layout for each sample is shown. The mean antibody concentration measured when each serum sample was incubated on BSA-coated wells, representing polyvalent antibody binding, was subtracted from the antibody concentrations measured on wells coated with the soluble Aβ conformations. After calculating the mean anti-monomer antibody concentration of each sample, 30% of this was subtracted from its antibodies to the oligomer preparation to determine its anti- oligomer antibody concentration. An IvIg sample (Gamunex) was included on all plates as a positive control. (CTL serum = normal control serum sample included on all plates to allow normalization of data between plates; Rush serum = experimental serum sample whose anti-Aβ antibody concentrations were being measured; GX = Gamunex Immune Globulin Intravenous (Human), 10%, Talecris Biotherapeutics, Inc., Research Triangle Park, NC).

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Journal of Neuroinflammation

ISSN: 1742-2094