Figure 2

Inhibition of NO, IL-1β and TNF-α production by ATL in LPS-stimulated BV-2 cells. BV-2 cells were pretreated with vehicle (0.035% ethanol) or various concentrations of ATL (1, 10 and 100 nM) for 30 min in the absence or presence of 100 μM Boc-2 (30 min before ATL treatment), a lipoxin receptor antagonist, followed by stimulated with LPS (100 ng/ml) for 24 h. (A) Nitrite content was measured using the Griess reaction. The concentration of IL-1β (B) and TNF-α (C) in culture media was measured using a commercial ELISA kit. (D) Cell viability was assessed by MTT assay, and the results are expressed as the percentage of surviving cells compared to control cells. Each value represents the mean ± SEM for three independent experiments. **P <0.01 compared with LPS in the absence of ATL; ^## P <0.01 compared with vehicle.