Figure 4

A specific ligand for DAI induces inflammatory cytokine production by isolated cultures of microglia and astrocytes. Microglia and astrocytes were untreated (0), treated with transfection reagent alone (FG), or transfected with the DAI ligand B-DNA (3 or 6 ug/ml). At 6 and 12 hours following transfection culture supernatants were collected and IL-6 and TNF-α content was assessed by specific capture ELISA. For comparison purposes inflammatory cytokine production was assessed at 24 hours following HSV-1 infection (MOI of 1 and 10). Data is expressed as mean +/- SEM (n = 6) and an asterisk indicates a statistically significant difference from levels produced by unstimulated cells (p < 0.05).