Figure 5

DAI knockdown attenuates HSV-1-induced inflammatory cytokine production by murine glial cells. Microglia and astrocytes were untreated (0), or transfected with siRNA targeting DAI (siDAI) or scrambled siRNA (siCon). At 72 hours following transfection, cells were exposed to HSV-1 (MOI of 0.01, 0.1, 1, and 10) and levels of TNF-α and IL-6 in the culture supernatants were assessed at 24 hours post-infection. Data is expressed as mean +/- SEM (n = 3) and an asterisk indicates a statistically significant difference from levels produced by cells treated with scrambled siRNA (p < 0.05).