Figure 4

Iba-1 ⁺ /CD45 ⁺ cells appear and express phagocytic activity in injured spinal cords. (A, B, E, F) Sections from intact, P1, or P2 regions at the indicated times after the injury were labeled with indicated antibodies. White arrows indicated positively labeled cells with each antibody. (B) Black arrows (P1, 7 d) indicated CD45⁺ cells that are not round. (C, D) Spinal cord tissues were obtained at the indicated times after SCI, and total RNA and protein were prepared and analyzed by RT-PCR (C) and Western blotting (D), respectively, as described in Materials and methods. Data shown are representative of at least three independent experiments. Scale bars: 20 μm.