WNT-5A induced a transient phosphorylation of ERK1/2. WNT-5A stimulation of mouse primary microglia induced ERK1/2 phosphorylation in a time- (A; 300 ng/ml WNT-5A) and dose-dependent (B; 30 minutes WNT-5A) manner. Bar graphs provide a summary of densitometric analysis of three independent experiments. Data were normalized to the response in ctrl-stimulated microglia. Variation is shown as s.e.m. (n ≥ 3). *, P < 0.05; **, P < 0.01; ***, P < 0.001. ctrl, control; ERK1/2, extracellular signal-regulated kinase 1/2; n, number; s.e.m, standard error of the mean.