Figure 4
From: Immunodominant fragments of myelin basic protein initiate T cell-dependent pain
Accumulation of MBP69-86 at the nodes/paranodes of myelinated fibers. Dual-immunostaining of MBP69-86 using a specific antibody (AB5864, Millipore, red, A-F) and MMP-9 (green, A-B), MHCII (green, C-E) or CD68 (green, F) in teased nerve fibers at day 1 (A-D) or week 1 (E-F) after and immediately proximal to transection. DAPI, blue. MMP-9 and MBP69-86 co-localize in the perinuclear and cytosolic regions of the myelinating Schwann cells (A). MMP-9, MBP69-86 and MHCII co-localize in the paranodal/nodal areas of presumably, A-fibers (arrows, B, C, D). Additionally, MBP69-86 is found in small, MHCII-positive cells adjacent to the fibers (E, arrowhead), such as CD68-reactive macrophages (F, arrowheads). Representative of approximately 20 individual fibers in n = 3/group. Scale bars, 10 μm.