Figure 3

Effects of indomethacin administration on Nogo-A expression. Animals were in one of four groups: sham (no TBI), TBI treatment (TBI eight hours), TBI combined with vehicle administration (TBI + vehicle), and TBI combined with indomethacin administration (TBI + indomethacin). (A) RT-PCR analysis of the expression of Nogo-A among different groups along with the analysis of β-actin transcription as an internal control. (B) Quantification of Nogo-A expression. (C) Western blot analysis of the expression of Nogo-A among different groups along with the analysis of β-actin as an internal control. (D) Quantification of Nogo-A expression. Bars represent means ± SEM values (n = 5). *P <0.05 is considered significantly different from the sham value using the Mann–Whitney U test and #P <0.05 is considered significantly different from the TBI value using the Mann–Whitney U test. SEM, standard error of the mean; TBI, traumatic brain injury.