CSF western blot analysis. (a) MMP-8 levels are elevated in injured animals (I) compared with controls (C) 12 days post-contusion (n = 4 for each condition). Rat lung lysate (L) was loaded as a positive control. Blots were probed for Transferrin as a loading control. (b) TIMP-1 is observed in over-expressing 293 cells (293) and rat brain lysate (B), but not in CSF CSF, cerebrospinal fluid; MMP, matrix metalloproteinase; TIMP-1, tissue inhibitor of metalloproteinase-1.