Figure 5
From: Efficient isolation of live microglia with preserved phenotypes from adult mouse brain
Analysis of the CD11b - (effluent) fraction. Cells in the effluent fraction were stained with anti-GFAP or anti-NeuN antibodies. (A) No CD11b+ cells were detected in this fraction, suggesting high efficiency of the CD11b+ cell separation from CNS tissues. As expected, this fraction consisted of large populations of (B) astrocytes and (C) neurons. (D) These observations were confirmed by mRNA analysis. High expression of CD11b was found only in the CD11b+ fraction, with undetectable levels in the effluent fraction, whereas high mRNA levels of GFAP and NeuN were found only in the effluent fraction.