Table 2 Comparison of the number of isolated live CD11b + cells determined by Trypan blue exclusion (n = 3)
From: Efficient isolation of live microglia with preserved phenotypes from adult mouse brain
Live cells (%) | Live cells/mg tissue | Live cells/brain | |
|---|---|---|---|
Percoll | 93 ± 0.9 | 2011 ± 167 | 994,000 ± 82,454 |
Anti-myelin beads | 81 ± 1.8 | 1792 ± 241 | 878,400 ± 110,963 |
Sucrose | 61 ± 4.4 | 884 ± 88 | 409,791 ± 53,427 |