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Table 2 Comparison of the number of isolated live CD11b + cells determined by Trypan blue exclusion (n = 3)

From: Efficient isolation of live microglia with preserved phenotypes from adult mouse brain

  Live cells (%) Live cells/mg tissue Live cells/brain
Percoll 93 ± 0.9 2011 ± 167 994,000 ± 82,454
Anti-myelin beads 81 ± 1.8 1792 ± 241 878,400 ± 110,963
Sucrose 61 ± 4.4 884 ± 88 409,791 ± 53,427