The enteric bacterial metabolite propionic acid alters brain and plasma phospholipid molecular species: further development of a rodent model of autism spectrum disorders

Table 1 Changes (percent composition) in rat brain sphingomyelin molecular species following intraventricular infusion with propionic acid (PPA) and phosphate buffered saline (PBS)

Molecular weight	Molecular species	Base/acyl species	PBS	PPA
703	34:1	18:1/16:0	0.07 ± 0.03	0.10 ± 0.05
733	36:0	18:0/18:0	21.96 ± 0.35	39.13 ± 1.35*
731	36:1	18:1/18:0	6.21 ± 0.67	4.53 ± 1.28
761	38:0	18:0/20:0	38.09 ± 0.84	29.37 ±1.92*
759	38:1	18:1/20:0	16.08 ± 0.19	12.31 ± 3.64*
789	40:0	18:0/22:0	13.03 ± 0.24	9.92 ± 1.31*
811	42:3	18:1/24:2	4.56 ± 0.12	4.64 ± 1.54
Total%			100	100
∑ Saturates			73.07 ± 0.15	78.41 ± 1.44*
∑ Monounsat			22.37 ± 0.66	16.94 ± 2.91*
∑ Polyunsat			4.55 ± 0.12	4.63 ± 1.54

Values (nanomole percent by weight composition) represent means ± standard errors. Means in the same row accompanied by asterisks are significantly different between treatments at LSD = 0.05, n = 12 per treatment group. Monounsat, monounsaturated fatty acids; PBS, phosphate buffered saline solution; PPA, propionic acid; polyunsat, polyunsaturated fatty acids. Sphingomyelin molecular species were identified using a precursor ion scan of m/z 184 in ESI positive mode. The lipid components in the table are arranged based on the molecular species composition.

ISSN: 1742-2094