The enteric bacterial metabolite propionic acid alters brain and plasma phospholipid molecular species: further development of a rodent model of autism spectrum disorders

Table 3 Changes (percent composition) in rat brain phosphatidylcholine molecular species following intraventricular infusion with propionic acid (PPA) and phosphate buffered saline (PBS)

Molecular weight	Molecular species	Plasmalogen or diacyl species	PBS	PPA
706	D30:0	16:0/14:0	0.13 ± 0.055	0.13 ± 0.04
734	D32:0	16:0/16:0	19.23 ± 0.34	19.33 ± 0.69
718	P32:1	p16:0/16:1	0.05 ± 0.01	0.06 ± 0.01
762	D34:0	16:0/18:0	18.66 ± 0.49	19.14 ± 0.63
760	D34:1	16:0/18:1	32.80 ± 0.15	33.68 ± 0.16*
746	P34:1	p16:0/18:1	1.54 ± 0.26	1.40 ± 0.32
758	D34:2	16:0/18:2	0.27 ± 0.04	0.69 ± 0.19*
788	D36:1	18:0/18:1	10.53 ± 0.18	9.20 ± 0.15*
774	P36:1	p18:0/18:1	0.50 ± 0.11	0.40 ± 0.11
782	D36:4	16:0/20:4	3.96 ± 0.51	4.81 ± 0.53*
814	D38:2	18:1/20:1	1.21 ± 0.25	1.29 ± 0.11
812	D38:3	18:0/20:3	1.85 ± 0.28	1.74 ± 0.14
810	D38:4	18:0/20:4	4.91 ± 0.26	4.33 ± 0.34
806	D38:6	16:0/22:6	2.98 ± 0.12	2.74 ± 0.10
834	D40:6	18:0/22:6	1.36 ± 0.11	1.06 ± 0.14
Total%			100	100
∑ Saturates			18.78 ± 0.48	19.27 ± 0.60
∑ Monounsat			62.56 ± 0.51	62.20 ± 0.70
∑ Polyunsat			16.55 ± 0.63	16.65 ± 0.99
∑ Plas			2.09 ± 0.32	1.85 ± 0.43

Values (nanomole percent by weight composition) represent means ± standard errors. Means in the same row accompanied by asterisks are significantly different between treatments at LSD = 0.05, n = 12 per treatment group. D represents diacyl species and P represents plasmalogens species, p denotes a sn-1 vinyl ether (plasmalogen) linkage. Monounsat, monounsaturated fatty acids; PBS, phosphate buffered saline solution; plas, plasmalogens; polyunsat, polyunsaturated fatty acids; PPA, propionic acid. Phosphatidylcholine molecular species were identified using a precursor ion scan of m/z 184 in ESI positive mode.

ISSN: 1742-2094