Table 2 Western Blot primary and secondary antibodies.
From: Activation of TRPV1 by capsaicin induces functional Kinin B1 receptor in rat spinal cord microglia
Primary antibodies | Molecular weight | Dilution | Source | ||
|---|---|---|---|---|---|
rabbit polyclonal anti-rat B1R | 37 kDa | 1:1000 | Réjean Couture's Laboratory [48] | ||
rabbit polyclonal anti-rat p65NF-κB | 65 kDa | 1:1000 | SantaCruz Biotechnology (Cat number Sc-8008) | ||
mouse monoclonal anti-rat dynein | 70 kDa | 1:20000 | SantaCruz Biotechnology (Cat umber Sc-13524) | ||
Secondary antibodies | Protein detected | Dilution | Source | ||
HRP-linked goat anti-rabbit | B1R, p65NF-κB | 1:5000 | SantaCruz Biotechnology (Cat number Sc-2077) | ||
HRP-linked goat anti-mouse | dynein | 1:5000 | SantaCruz Biotechnology (Cat number Sc-2005) | ||