Figure 1

Inhibition of microglial and astrocytic NO production by hypothermia. BV-2 microglial cells (A), primary microglia cultures (B), primary astrocyte cultures (C) (5 x 10⁴ cells per well in a 96-well plate) were incubated with 100 ng/ml of LPS under hypothermic (33 °C, 29 °C) or normothermic (37 °C) conditions for 0 to 72 hours. The amounts of nitrite in the culture media were measured with the Griess reagent (upper). Cell viability was examined by MTT assays and the results were expressed as the percentage of surviving cells over the controls (lower). Results are the mean ± SD (n = 3). *P < 0.05, **P < 0.01; significantly different from the LPS-treated group at the same time point under normothermic condition (37 °C). LPS, lipopolysaccharide; MTT, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide; n, number.