Figure 12

Protective effect of hypothermia against microglial neurotoxicity in primary microglia and cortical neuron co-cultures. Co-cultures of primary microglia and cortical neurons were done using culture inserts as shown (A, B). Following LPS stimulation of the primary microglia/neuron co-cultures under normothermic or hypothermic condition, the co-cultures were further incubated for 48 hours. Afterwards, the culture inserts containing microglial cells were removed, and a MTT assay was performed to determine the viability of cortical neurons (C). Results are the mean ± SD (n = 3). **P < 0.01; significantly different from the LPS-treated group under normothermia. LPS, lipopolysaccharide; MTT, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide; n, number; O/N, overnight.