Figure 15

Effect of hypothermia on microglial cell migration: the Boyden chamber assay. Primary microglial cultures (A) or BV-2 microglial cells (1 x 10⁴ cells per well) (B) were seeded on the upper compartment of the Boyden chamber. After three hours (BV-2 microglial cells) or six hours (primary microglia) incubation under normothermic (37 °C) or hypothermic (33 C, 29 C) conditions, cells that migrated through the membrane were stained (A upper) and counted to evaluate the relative cell migration (A lower, B). A representative microscopic image for each condition is shown (magnification, x40 or x200) (A upper). Scale bar = 200 μm. Results are the mean ± SD (n = 3). **P < 0.01, *P < 0.05; significantly different from normothermia (37 C). n, number.