Figure 8

Effect of hypothermia on ROS production in LPS-stimulated microglial cells: DCFDA assay. BV-2 microglial cells (5 x 10⁴ cells per well in a 96-well plate) were treated with LPS (100 ng/ml) for six hours under normothermic (37 °C) or hypothermic (29 °C) conditions as indicated (A). Production of ROS was evaluated by using DCFDA staining (B). Results are mean ± SD (n = 3). *P < 0.05, **P < 0.01; significantly different from LPS treatment under normothermia. DCFDA, 2'-7'-dichlorodihydrofluorescein diacetate; LPS, lipopolysaccharide; n, number; ROS, reactive oxygen species.