Figure 7

C/EBPβ binding to CD200R1 gene promoter in glial cells. (A) Analysis of C/EBPβ binding to six putative binding sites of the CD200R1 gene promoter in primary mixed glial cultures by qChIP. Significant binding of C/EBPβ was only observed in box 6 in LPS-treated cultures (four hours). Bars are means + SEM of three independent experiments. ***P <0.001 versus IgG and C. One-way ANOVA and Bonferroni post-test. (B) C/EBPβ binding to box 6 of the CD200R1 gene promoter in BV2 pCDNA and BV2-LAP cells. Significant binding of C/EBPβ was observed in LPS-treated (four hours) BV2-pCDNA cells but also in control BV2-LAP cells. LPS treatment did not induce a further increase in C/EBPβ binding in LPS-treated BV2-LAP cells. Bars are means + SEM of three independent experiments. ***P <0.001 versus IgG and C; ##P <0.01 versus the equivalent experimental condition in BV2-pCDNA cells. Two-way ANOVA and Bonferroni post-test. ANOVA, analysis of variance; C, control; C/EBPβ, CCAAT/enhancer binding protein β; IgG, immunoglobulin G; LPS, lipopolysaccharide; qChIP, quantitative chromatin immunoprecipitation; SEM, standard error of the mean.