Figure 4

Increased hippocampal neurogenesis in runners seven weeks after bacterial meningitis. Detection of NeuN (A) and BrdU (B) by double-label fluorescent immunohistochemistry. Merger of both markers revealed a newly formed neuron 7 weeks after bacterial meningitis (C). There was a higher percentage of co-labeled BrdU + NeuN + neurons in running mice in comparison to sedentary controls (D) (median and interquartile range, P = 0.018, Mann–Whitney U-test, scale bar = 50 μm). BrdU: Bromodeoxyuridin; NeuN: Neuron-specific nuclear antigen.