Figure 5From: Delayed expression of cell cycle proteins contributes to astroglial scar formation and chronic inflammation after rat spinal cord contusion The later upregulation of cell cycle proteins is associated with activated microglial/macrophages. The expression of PCNA, cyclin D1 and E was evaluated by immunohistochemistry at 1.5 mm caudal to the epicenter of sham or injured spinal cord at 1 month post-injury. (A-D) PCNA expression was undetectable in sham, but was strongly upregulated in OX42+ microglia/macrophages. (E-H) The membrane bound component of the NADPH oxidase enzyme, p22, co-localized with PCNA+ microglia/macrophages. (I-P) Large numbers of cyclin E+ and D1+ cells in the injured coronal sections were co-labeled with Iba-1+ or OX42+ microglia/macrophages. Scale bars = 500 μm.Back to article page