Figure 8
Effects of sPLA 2 -IIA inhibitor, NOS inhibitors, and STAT1 siRNA on the permeability of BMVECs after LPS treatment. Cells were pretreated with the sPLA2-IIA inhibitors S3319 (2 μM) and Ly311727 (10 μM); the NOS inhibitors L-NAME (1 mM) and AG (1 mM); the NOS agonist, SNP (1 μM); JAK3 (10 μM); the STAT1 inhibitors, WHI (10 μM) and Flu (10 μM); and STAT1 siRNA; and then the BMVECs were stimulated by 5 μg/ml LPS for 16 h. BMVEC permeability was analyzed by the effusion capacity of biotin-BSA from the cell monolayers. Quantitative data of the cell permeability after LPS and pretreatment with L-NAME, AG, SNP, WHI, Flu, or siRNA STAT1. The data are presented as the means ± SE of five separate experiments. *P < 0.05 versus the normal group, ^ P < 0.05 versus LPS treatment alone group.