Figure 2

Changes in FasL expression after microsphere embolism induction in the brain. (A) Representative immunoblots showing Fas and FasL expression at the indicated times following microsphere embolism in rats. Immunoblotting with an anti-β-actin antibody showed equal total protein loading in each lane. S, sham. (B) Quantitative analysis of Fas and FasL levels was performed by densitometric analysis of the immunoblots. The data are expressed as percentages of values obtained from sham animals (mean ± SEM, n  = 6 rats). * P <0.05; **P <0.01 vs. sham. (C) Fluorescent staining for FasL (green) and NeuN (red) was performed in ipsilateral brain regions 168 h after embolic injury in rats. (D) FasL (green, a, d) and GFAP (red, b, e) double staining was performed 168 h after embolic injury in rats. (E) Immunohistochemical localization of FasL (green) and ED1 (red) was examined 168 h after ME ischemia. Scale bar = 40 μm. (F) Quantification of colocalized FasL-NeuN, FasL-GFAP, and FasL-ED1 fluorescent signals in rats following ME injury. The mean overlapping fraction, the mean Pearson’s r and the mean ICQ are presented for each condition (the mean ± SEM, n = 6 rats). ME, microsphere embolism.