Table 1 Primer sequences
From: Acute injury in the peripheral nervous system triggers an alternative macrophage response
Negative regulators | ||
|---|---|---|
A20 | GAACAGCGATCAGGCCAGG | GGACAGTTGGGTGTCTCACATT |
IκBα | TGAAGGACGAGGAGTACGAGC | TTCGTGGATGATTGCCAAGTG |
IL-1RA | GCTCATTGCTGGGTACTTACAA | CCAGACTTGGCACAAGACAGG |
MyD88s | TGAAGTCGCGCATCGGAC | CGGCGACACCTTTTCTCAAT |
SIGIRR | GTGACATGGCCCCTAATTTCC | ATGCCAGACCATCTTTCAGCC |
SOCS1 | CTGCGGCTTCTATTGGGGAC | AAAAGGCAGTCGAAGGTCTCG |
Immune mediators | ||
Cox2 | TGAGCAACTATTCCAAACCAGC | GCACGTAGTCTTCGATCACTATC |
MIP-1α | TTCTCTGTACCATGACACTCTGC | CGTGGAATCTTCCGGCTGTAG |
IL1β | GCAACTGTTCCTGAACTCAACT | ATCTTTTGGGGTCCGTCAACT |
IL6 | TAGTCCTTCCTACCCCAATTTCC | TTGGTCCTTAGCCACTCCTTC |
MCP-1 | TTAAAAACCTGGATCGGAACCAA | GCATTAGCTTCAGATTTACGGGT |
Macrophage markers and M1/M2 markers | ||
AIF1/Iba1 | ATCAACAAGCAATTCCTCGATGA | CAGCATTCGCTTCAAGGACATA |
Arg1 | TGGCTTGCGAGACGTAGAC | GCTCAGGTGAATCGGCCTTTT |
CD11b | CCATGACCTTCCAAGAGAATGC | ACCGGCTTGTGCTGTAGTC |
Cdh1 | GTCTACCAAAGTGACGCTGAA | GGGTACACGCTGGGAAACAT |
F4/80 | ATGGACAAACCAACTTTCAAGGC | GCAGACTGAGTTAGGACCACAA |
FIZZ1 | CCAATCCAGCTAACTATCCCTCC | ACCCAGTAGCAGTCATCCCA |
IFNγ | ATGAACGCTACACACTGCATC | CCATCCTTTTGCCAGTTCCTC |
IL4 | GGTCTCAACCCCCAGCTAGT | GCCGATGATCTCTCTCAAGTGAT |
IL10 | ATGGCCGGATAGCCTTATTC | ACGATGTGGTGACAACCGTA |
IL12p40 | AGTGTGAAGCACCAAATTACTCC | CCCGAGAGTCAGGGGAACT |
IL-13 | GCAACATCACACAAGACCAGA | GTCAAGGAATCCAGGGCTAC |
iNOS | GTTCTCAGCCCAACAATACAAGA | GTGGACGGGTCGATGTCAC |
MRC1 | CTCTGTTCAGCTATTGGACGC | CGGAATTTCTGGGATTCAGCTTC |
TREM2 | GAACCGTCACCATCACTCTGA | CCTCGAAACTCGATGACTCCT |
Ym1 | AGAAGGGAGTTTCAAACCTGGT | CTCTTGCTGATGTGTGTAAGTGA |
IFNγR1 | GTGGAGCTTTGACGAGCACT | ATTCCCAGCATACGACAGGGT |
IL4Rα | ATTTTGCTGTTGGTGACTGGA | CGTGGAAGTGCGGATGTAGT |
IL10R | CCTATCCCAAACCAGTCTGAGA | CCAGGTTGAGTTTCCGTACTGT |
IL13Rα1 | TCAGCCACCTGTGACGAATTT | TGAGAGTGCAATTTGGACTGG |
Scavenger receptors | ||
Clec7a | ATTTTGGCGACACAATTCAGGG | GCAAGACTGAGAAAAACCTCCT |
Rage | ATGCACAGAAACGGGATCTTT | CTGCTTGGAATAGACACTCCG |
Housekeeping genes | ||
ACTB | GCTTCTAGGCGGACTGTTACTGA | GCCATGCCAATGTTGTCTCTTAT |
B2M | ATGCACGCAGAAAGAAATAGCAA | AGCTATCTAGGATATTTCCAATTTTTGAA |
HMBS | GAAACTCTGCTTCGCTGCATT | TGCCCATCTTTCATCACTGTATG |
RPL13a | CCTGCTGCTCTCAAGGTTGTT | TGGTTGTCACTGCCTGGTACTT |
TBP | TCTACCGTGAATCTTGGCTGTAAA | TTCTCATGATGACTGCAGCAAA |