Figure 6
From: C/EBPβ regulates multiple IL-1β-induced human astrocyte inflammatory genes
p38K-selective small molecule inhibitor blocks IL-1β-induced COX-2 expression. (A-D) Astrocytes were cultured in 48-well plates for 24 h, treated with selective inhibitors (SB203580-20 μM, and U0126-20 μM) for 1 h and then IL-1β (20 ng/ml) for 24 h. Cells were fixed, blocked and then incubated with antibodies against GFAP (green) and COX-2 (red). All pictures were taken at ×200, and data represent at least three independent experiments in at least two independent donors.