Figure 5

Caspase-3 expression is reduced in Thy1-HexB;HexB ^−/− mice. Caspase-3 immunoreactivity was employed as a measure of neurodegeneration. (A) Thy1-HexB;HexB^−/− mice lacked any detectable caspase-3 expression, as assessed by immunohistochemistry. (B) Conversely, HexB^−/− mice displayed intense caspase-3 immunoreactivity located in cortical neurons. (C) Wild-type mice did not present any caspase-3 immunostaining in their brain. (D) Cell counts of caspase-3 immunopositive cells was reduced significantly in Thy1-HexB;HexB^−/− mice, to levels observed in wild-type mice. Bar = 100 μM. ***P <0.001; **P <0.01; *P <0.05.