Figure 4
CCL2 enhances capsaicin currents and upregulates TRPV1 mRNA expression in neurons via activating Akt. (A) In the presence of Akt inhibitor IV (1 μM), chemokine CC chemokine ligand 2 (CCL2) (5 nM) pretreatment did not augment the magnitude of capsaicin (0.3 μM)-evoked inward current. Holding potential (VH) = −60 mV. (B) Akt inhibitor IV significantly blocked CCL2 upregulation of density of capsaicin currents. Each bar shows the mean ± standard error (SE) value of 10 neurons. (C) CCL2 pretreatment of dorsal root ganglion (DRG) neurons for 24 to 36 hours failed to increase transient receptor potential vanilloid receptor 1 (TRPV1) mRNA level in the presence of 1 μM Akt inhibitor IV. Each bar represents the mean ± SE value of five experiments. *P <0.01 compared with control neurons.