Figure 5
From: Podosomes in migrating microglia: components and matrix degradation
Microglial podosomes are enriched in Tyr 14 -phosphorylated caveolin-1 and nicotinamide adenine dinucleotide phosphate oxidase 1(Nox1). Deconvolved, color-separated and merged images of the lamellar region are shown for two microglia. The boxed areas are magnified in the images at the bottom. Scale bar: 5 μm (upper), 2 μm (lower). (A) Immunolabeling for tyrosine-phosphorylated caveolin-1 (p-Tyr14Cav1; green) and the podosome ring marker talin (red). (B) Immunolabeling for Nox1 (green) and talin (red). Note: Nox1 staining required methanol fixation.