Figure 7

ApoC-I suppresses Aβ-stimulated cytokine secretion from microglia. Cultured WT primary murine microglia were treated with a combination of fibrillar Aβ_1-42 (5 μM) and IFNγ (10U/ml) for 18 h and supernatant amounts of IL-6 and TNF-α (ng/μg protein) quantified by ELISA. Aβ/IFNγ induced robust secretion of IL-6 and TNF-α by cultured microglia into the extracellular media, an effect that was significantly reversed with co-administration of apoC-I (1 μM). Unstimulated vehicle controls were below the level of detection. Data are expressed as mean ± standard error of the mean (SEM) cytokine amount; n = 3. ***P < 0.001, Student’s t-test. (B,C) ApoC-I suppression of Aβ-stimulated secretion of IL-6 and TNF-α by microglia was unchanged by co-administration with RAP (1 μM). WT microglia were treated with Aβ_1-42 and IFNγ for 18 h in the presence of apoC-I (1 μM) and/or RAP (1 μM) and cytokine secretion measured by ELISA. Data are expressed as mean ± SEM percentage of maximum PIC stimulation; n = 3.