|
Protein
|
Hippocampal expression (pg/ml)
|
Cortical expression (pg/ml)
|
P-value
|
|---|
|
Chemokines
|
Cntrl
|
mTLE-HS
|
mTLE + HS
|
Cntrl
|
mTLE-HS
|
mTLE + HS
| |
|---|
|
CCL22/MDC
|
0.3
|
(0–0.7)
|
0.8
|
(0.4-1.6)
|
0.9
|
(0.3-1.4)
|
0.2
|
(0–0.6)
|
0.6
|
(0.3-1.2)
|
0.9
|
(0.7-1.3)
|
1.74E-08
|
|
CCL5/RANTES
|
8.1
|
(4–15.8)
|
77.4
|
(40.9-238.1)
|
49.4
|
(18.5-167.7)
|
10.9
|
(1.6-18.5)
|
26.0
|
(18.5-122.3)
|
31.5
|
(17.4-38.5)
|
1.60E-08
|
|
CCL4/MIP1β
|
0
|
(0–0)
|
19.4
|
(4–122.3)
|
18.9
|
(6.2-95.3)
|
0
|
(0–0)
|
0
|
(0–7.6)
|
0.0
|
(0–33.6)
|
2.11E-08
|
|
CXCL9/MIG
|
3.2
|
(1.1-11)
|
9.0
|
(6.7-16.7)
|
11.0
|
(4.4-17.1)
|
5.7
|
(2.4-7.7)
|
8.3
|
(5.7-12)
|
10.7
|
(7.0-12.3)
|
5.99E-07
|
|
CCL2/MCP1
|
1.1
|
(0–4.3)
|
11.4
|
(2.1-52.8)
|
9.3
|
(2.1-29.0)
|
0.0
|
(0–2.9)
|
0.7
|
(0–4.6)
|
0.3
|
(0–18.2)
|
4.13E-06
|
|
CCL3/MIP1α
|
44.4
|
(11.2-106.2)
|
94.5
|
(62–177.4)
|
94.6
|
(62–317.1)
|
35.6
|
(20.1-57.4)
|
62.0
|
(35.6-104.9)
|
85.1
|
(59.0-97.2)
|
9.13E-05
|
|
CCL19/MIP3β
|
0
|
(0–0)
|
0.5
|
(0–1.6)
|
0
|
(0–1)
|
0
|
(0–0)
|
0
|
(0–1.7)
|
0.4
|
(0–1.3)
|
9.90E-05
|
|
CCL18/PARC
|
16.6
|
(0.2-151.6)
|
24.2
|
(1.8-177.4)
|
14.0
|
(0–29.5)
|
13.7
|
(0–132.7)
|
3.3
|
(0–31.9)
|
0.7
|
(0–37.6)
|
0.001966
|
|
CXCL8/IL-8
|
0.8
|
(0–13.8)
|
2.7
|
(0–26)
|
1.0
|
(0.3-20.1)
|
0.6
|
(0–1.6)
|
0.5
|
(0–10.3)
|
0.9
|
(0–3.6)
|
0.02352
|
|
CCL11/Eotaxin
|
0
| |
0
| |
0
| |
0
| |
0
| |
0
| |
b.d.
|
|
CCL17/Tarc
|
0
| |
0
| |
0
| |
0
| |
0
| |
0
| |
b.d.
|
|
CXCL10/IP-10
|
0
| |
0
| |
0
| |
0
| |
0
| |
0
| |
b.d.
|
|
Cytokines
| | | | | | | | | | | | | |
|
IL-7
|
0
|
(0–5.6)
|
8.3
|
(3.8-9.6)
|
7.3
|
(0.6-12.2)
|
0.9
|
(0.0-4.6)
|
8.7
|
(4.6-14.5)
|
11.0
|
(8–12.7)
|
5.07E-15
|
|
IL-13
|
2.6
|
(0.9-6.8)
|
13.4
|
7.9-20.5)
|
11.2
|
(8.1-17.4)
|
6.4
|
(2.6-12.5)
|
12.7
|
(10.3-17.4)
|
15.4
|
(10.7-16.5)
|
7.60E-13
|
|
IL-22
|
92.5
|
(58.3-212.2)
|
351.2
|
(236.6-540.6)
|
304.1
|
(244.8-449.9)
|
212.2
|
(122.8-359.4)
|
380.0
|
(293.8-548.8)
|
425.2
|
(261.1-499.3)
|
1.65E-12
|
|
IL-5
|
0
|
(0–1.2)
|
2.8
|
(1.5-7.4)
|
2.8
|
(0.3-5.5)
|
0
|
(0–1)
|
2.3
|
(1–6.7)
|
5.0
|
(2–6.1)
|
2.06E-07
|
|
IL-25/IL-17E
|
726.9
|
(273.4-1780)
|
868.3
|
(348.3-1616)
|
1976.4
|
(258.5-2499)
|
303.3
|
(109.9-757.4)
|
673.5
|
(483.8-1234.5)
|
922.1
|
(468.7-1327.5)
|
1.93E-05
|
|
IL-1RA
|
13.5
|
(6.7-26.8)
|
19.0
|
(13.5-33.4)
|
20.7
|
(12.3-29)
|
15.7
|
(13.5-31.2)
|
29.0
|
(13.5-165.2)
|
53.1
|
(26.8-92.2)
|
2.96E-05
|
|
IL-1a
|
0
|
(0–0.2)
|
0.3
|
(0–3)
|
0.3
|
(0–1.3)
|
0
|
(0–0)
|
0
|
(0–0.9)
|
0.3
|
(0–1.6)
|
5.06E-05
|
|
IL-27
|
40.9
|
(0–153.8)
|
33.7
|
(0–133.4)
|
132.1
|
(0–370.5)
|
0
|
(0–42.3)
|
28.6
|
(0–89.7)
|
49.4
|
(29.4-78.7)
|
8.93E-05
|
|
IL-10
|
2.2
|
(0–5.9)
|
1.4
|
(0–3.4)
|
7.8
|
0-12.4)
|
0
|
(0–2.2)
|
0.5
|
(0–1.9)
|
0.9
|
(0–3.4)
|
1.02E-04
|
|
IFNα
|
22.5
|
(12.8-41.5)
|
31.5
|
(22.7-58.5)
|
66.3
|
(10.1-114.3)
|
27.0
|
(4.1-73.1)
|
41.3
|
(26.9-51.5)
|
38.5
|
(30.7-59)
|
3.40E-04
|
|
MIF
|
11135.4
|
(7054–13156)
|
8200.7
|
(6406–19843)
|
6907.3
|
(4910–9650)
|
11062.2
|
(4645–17980)
|
7112.0
|
(4645–9872)
|
7180.8
|
(5547–9470)
|
0.0024
|
|
IL-1β
|
0.2
|
(0–0.9)
|
0.8
|
(0.2-1.7)
|
0.9
|
(0–1.7)
|
0.2
|
(0–0.9)
|
0.5
|
(0–1.5)
|
0.9
|
(0–1.7)
|
0.0063
|
|
IL-23
|
298.9
|
(190.4-568.7)
|
575.1
|
(473.2-675)
|
556.2
|
(311.2-691)
|
308.1
|
(238.2-717)
|
500.2
|
(286.7-633)
|
543.2
|
(372.9-710)
|
0.0063
|
|
IL-4
|
0
|
(0–0.1)
|
0.1
|
(0–0.2)
|
0
|
(0–0.2)
|
0
|
(0–0)
|
0
|
(0–0.2)
|
0.1
|
(0–0.2)
|
0.0234
|
|
IL-6
|
0
|
(0–11.7)
|
0
|
(0–3.9)
|
0
|
(0–1.1)
|
0
|
(0–2.1)
|
0
|
(0–0)
|
0.0
|
(0–0)
|
0.0428
|
|
IL-21
|
336.5
|
(117.7-944.2)
|
510.6
|
(0–944.2)
|
831.0
|
(55.8-1270.4)
|
431.2
|
(0–1009.1)
|
415.4
|
(0–847.1)
|
654.1
|
(179.9-1534)
|
0.0848
|
|
TNFα
|
10.4
|
(8.6-12.7)
|
11.5
|
(7.0-13.2)
|
11.2
|
(8.2-13.2)
|
9.3
|
(6.3-12.9)
|
10.6
|
(6.9-12.3)
|
10.5
|
(9.2-13.2)
|
0.1341
|
|
IL-18
|
3.7
|
(1.7-14.2)
|
3.2
|
(1.5-5.2)
|
2.1
|
(1.1-23.8)
|
5.0
|
(1.6-163.1)
|
2.9
|
(1.7-12.4)
|
2.9
|
(2.2-4.8)
|
0.1481
|
|
IL-6R
|
1.4
|
(0–10)
|
1.4
|
(0–5.6)
|
4.5
|
(1.4-8.9)
|
3.4
|
(0–17)
|
3.4
|
(0–10)
|
4.5
|
(0–7.8)
|
0.4944
|
|
IL-2
|
0
| |
0
| |
0
| |
0
| |
0
| |
0.0
| |
b.d.
|
|
IL-12p70
|
0
| |
0
| |
0
| |
0
| |
0
| |
0.0
| |
b.d.
|
|
Other
| | | | | | | | | | | | | |
|
VCAM1
|
219.7
|
(33.9-828.3)
|
985.5
|
(548–1722.6)
|
1181.3
|
(364.5-1649)
|
210.3
|
67.3-527.2)
|
817.5
|
(444.9-1287)
|
1239.9
|
(740.4-1551)
|
2.71E-11
|
|
VEGF
|
10.0
|
(0.4-28.8)
|
30.3
|
(18.6-47)
|
31.1
|
(7.2-59.3)
|
4.5
|
(0–12.8)
|
22.9
|
(12.8-39.3)
|
32.6
|
(17.1-36.3)
|
3.70E-10
|
|
HGF
|
33.0
|
(26.7-54.5)
|
105.2
|
(65.8-157.4)
|
90.3
|
(34.6-156.4)
|
24.0
|
(19–50.3)
|
71.3
|
(37–207.7)
|
52.4
|
(32.2-83.6)
|
1.38E-07
|
|
ICAM1
|
9099.6
|
(3364–17901)
|
5400.2
|
(3174–9985)
|
16011.1
|
(5781–26620)
|
5039.5
|
(1532–16739)
|
3440.4
|
(1532–10754)
|
4698.9
|
(1861–8725)
|
9.13E-05
|
|
Cathepsin S
|
463.3
|
(284.2-1010)
|
311.9
|
(129.1-525.7)
|
218.0
|
(160.4-425.5)
|
350.6
|
(198.2-993.6)
|
303.8
|
(198.2-478.7)
|
327.2
|
(190.4-413)
|
0.0025
|
|
TIMP-1
|
101.0
|
(36.2-2243.7)
|
124.3
|
(81.6-382.5)
|
103.2
|
(50.7-204)
|
81.6
|
(53.7-137.7)
|
97.7
|
(64.5-324.4)
|
112.5
|
(83.5-305.6)
|
0.2672
|
|
Adiponectin
|
415.0
|
(14–1412.5)
|
727.8
|
(193.3-1643)
|
480.3
|
(221–1787.4)
|
722.4
|
(22.4-1979.9)
|
490.0
|
(22.4-940.7)
|
349.3
|
(0–1751)
|
0.4253
|
- MIA was performed on selected chemokines, cytokines, growth factors and adhesion molecules. HC and CX protein levels were determined in controls and 2 mTLE patient groups (− and + HS).Inflammatory proteins are listed in order of overall significance (ANOVA/Kruskal-Wallis). Proteins in bold show significant changes between groups (ANOVA/Kruskal-Wallis, p < 0.0015). Levels are presented as medians and range (between brackets). Abbreviations: b.d. below detection, HGF hepatocyte growth factor, ICAM1 intercellular cell adhesion molecule 1, IFNα interferon type 1α, IP10 Interferon gamma-induced protein 10, MCP1 macrophage derived chemokine, MDC macrophage derived chemokine, MIF Macrophage migration inhibitory factor, MIG monokine induced by interferon γ, MIP1α Macrophage inflammatory protein-1α, MIP1β Macrophage inflammatory protein-1β, MIP3β Macrophage inflammatory protein-3β, PARC pulmonary and activation-regulated chemokine, RANTES Regulated upon activation normal T cell express sequence, TARC thymus and activation regulated chemokine, TIMP-1 tissue inhibitor of metalloproteinases, TNFα tumor necrosis factor, VCAM1 vascular cell adhesion molecule 1, VEGF vascular endothelial growth factor.
