Figure 3

Systemic inflammation activates microglia and enhances COX-2 activity in RVLM. Gels (inset) and densitometric analysis of results from Western blot showing changes in expression of Iba-1 (A) or COX-2 (C), or representative photomicrographs showing immunoreactivity to Iba-1 (black color) (B), COX-2 activity (D), or tissue level of PGE2 (E) in RVLM, examined on day 7 after intraperitoneal infusion of saline or LPS (1.2 mg/kg/day), alone or with additional intracisternal infusion of minocycline (9 nmol/μL/). Values are mean ± SEM (n=8 to 10 animals in each experimental group). *P <0.05 vs. saline-treatment group; ^# P <0.05 vs. LPS-treatment group in the post hoc Scheffé multiple-range test. Scale bar in (A): 25 μm.