Supernatant derived from MAPC:splenocyte
co-cultures promotes apoptosis of
proinflammatory microglia/macrophages. (A) Triple positive microglia (CD86+/CD206+/CC3+) significantly decreased after treatment with supernatant derived from MAPC:splenocyte co-cultures (P ≤0.01). (B) A significant increase in M1 microglial apoptosis was measured by the number of CD86+/CC3+ microglia in microglia treated with supernatant derived from MAPC:splenocyte co-cultures (P ≤0.05). (C) No significant difference in the number of CD206+/CC3+ microglia between the two groups was observed. (D) Photomicrograph of isolated microglia/macrophages activated LPS and splenocyte alone (without MAPC) supernatant. Note the number of triple stained cells. (E) Photomicrograph of isolated microglia/macrophages activated LPS and incubated with MAPC in direct contact with splenocytes supernatant treatment. Note the number of M1/CC3 as indicated by arrows. M2 positive cells are labeled green, CC3 positive cells are labeled red, and M1 positive cells are labeled blue * and ** represents P <0.05. LPS, lipopolysaccharide; MAPCs, multipotent adult progenitor cells.