Figure 3

Immunohistochemistry and autoradiography of ¹³¹ I-lymphocytes. Cervical lymph node lymphocytes from SJL or CD-1 mice were radioactively labeled and injected intravenously into the autologous strain. After 30 min, cervical nodes or brain tissue were harvested, processed, and stained for CD45. Panel A shows ¹³¹I-lymphocyte preparation of cervical lymph node lymphocytes obtained from SJL female mice stained for CD45. Panel B shows stained ¹³¹I-lymphocytes prepared from the cervical lymph nodes of CD-1 males. Panels C and D show CD-1 cervical lymph node cells 30 min after the i.v. injection of ¹³¹I-lymphocytes. Panel E shows stained ¹³¹I-lymphocyte in cortex from SJL mouse. Panel F shows stained ¹³¹I-lymphocyte from hindbrain in SJL mouse. Panels G and H show CD45 stained ¹³¹I-lymphocytes from occipital cortex of CD-1 mice. All magnifications are at 40×.