Electrophysiological analysis of excitability of peripheral afferent fibers in the skin nerve preparation in SJL-PLP
peptide immunized mice and control mice. Shown are electrophysiological recordings of firing rates evoked by application of increasing 10 seconds lasting pressure via a nanomotor (expressed in terms of displacement) from (A) Aδ-type of mechanonociceptors, (B) C-mechanonociceptors (C-fibers), (C) Aβ low threshold mechanoceptors, rapidly adapting (RA), and (D) slowly adapting (SA), in the skin-nerve preparation derived from the paws of control mice (black symbols) and SJL-EAE mice (red symbol) in the chronic phase of the disease (days 35 to 45). (E) Proximal nerve fiber velocities were calculated from the action potential latency to the electrical stimulation and distance of the receptive field from the measuring electrode. (F) Mechanical threshold to the accidently applied calibrated von Frey filaments. *indicates significant statistical difference (P <0.05; ANOVA followed by post-hoc Bonferroni’s test). n represents the number of fiber type for each tested animal group. EAE, experimental autoimmune encephalomyelitis.