Figure 8

Astrocyte and microglia expression in the spinal dorsal horn of control immunized mice and SJL-PLP _139-151 peptide immunized mice over the time course of the EAE. (A) Representative examples of GFAP expression at 2 to 3 days following immunization (pre-timepoint), in the onset phase, in the peak phase and in the chronic phase of the disease (day 35 to 40 after immunization). There was a significant increase in GFAP positive cells over time in SJL-EAE mice. (B) Representative examples of Iba1 positive cells at 2 to 3 days following immunization (pre-timepoint), in the onset phase, in the peak phase and in the chronic phase of the disease (day 35 to 40 after immunization). The expression of Iba1 increased significantly over time in SJL-EAE mice in the spinal dorsal horn. Scale bars represent 100 μm in panel A and B. EAE, experimental autoimmune encephalomyelitis.