Effect of OGD/reperfusion on NO production and iNOS expression.
A. The changes of NO levels in cultured astrocytes following OGD/reperfusion as determined by Griess reagent. Following reperfusion, NO production increased gradually and reached a peak under the treatment of OGD 8 h/reperfusion 24 h. This increase was significant in comparison with all other groups. B. iNOS protein levels in cultured astrocytes following OGD/reperfusion as determined by Western blotting. Full-length iNOS protein (~130 kDa) was more enriched in the OGD/reperfusion groups compared with the control group. OGD/reperfusion significantly induced greater levels of iNOS than in normal conditions. Five independent experiments were performed and data were analyzed in triplicate. Values were presented as mean ± SEM; *, P < 0.05; **, P < 0.01; ***, P < 0.001 by one-way ANOVA (analysis of variance) with Tukey’s post-hoc test.