Distribution of ubiquitin-conjugated proteins and SOD1 in cultured astrocytes following OGD/reperfusion.
A. In astrocytes, the ubiquitin immunoreactivity in the normoxic control group was evenly distributed in cytoplasm and nucleus. Following OGD 8 h/reperfusion 16 h, the diffused distribution of free ubiquitin lost nuclear staining and clustered immunoreactivity appeared near the nucleus. 1400W attenuated the punctuated ubiquitin perinuclear distribution B. Colocalization of ubiquitin and SOD1. Following OGD 8 h/reperfusion 16 h, were identified. SOD1 immunoreactivity colocalized with ubiquitinated-protein aggregates and showed a pattern of punctuated perinuclear-nuclear localization. Scale bar, 100 μm; green, ubiquitin immunosignals; red, SOD1 immunosignals; blue, nucleus staining with Hoechst 33342.