Figure 10

Representative images of glial fibrillary acidic protein (GFAP) immunostaining in C3H/HePas wild-type (WT) and C3H/HeJ Toll-like receptor (TLR)4 mutant mice 1 week after unilateral axotomy. (A, C) Contralateral side and (A,C) ipsilateral side of the of the lumbar spinal cord of C57BL/6J WT and TLR2^−/− mice. The lesion upregulated GFAP labeling in both groups, with no differences between groups. (E) Graph representing the ipsilateral:contralateral ratio of the integrated density of pixels. (F,G) Purified astrocyte cultures showing astroglial reactivity in C3H/HePas WT and C3H/HeJ TLR4 mutant cultures. (H) Graph showing no significant difference in GFAP immunolabeling between these two groups. Scale bar: 50 μm.