Figure 11

Cell proliferation assay in purified astrocyte cultures from C3H/HePas wild-type (WT) and C3H/HeJ Toll-like receptor (TLR)4 mutant mice. (A-R) 4^′,6-diamidino-2-phenylindole (DAPI and 5-bromo-2^′-deoxyuridine (BrdU) immunostaining after culturing for 3 days. (A-L) Single labeling with (A-F) DAPI and (G-L) BrdU. (M-R) Double labeling with DAPI and BrdU. (S) Graph showing the ratio of BrdU to DAPI; there was no difference in the proliferation rate between WT and TLR4 mutant during 3 days of culturing. (T) Graph showing the astrocyte number for each day; TLR4 signaling did not affect astrocyte proliferation. Scale bar: 50 μm.