Cell proliferation assay in purified astrocyte cultures from C57BL/6J wild-type (WT) and Toll-like receptor (TLR)2 knockout (KO) mice (TLR2
) mice. (A-R) 4′,6-diamidino-2-phenylindole (DAPI and 5-bromo-2′-deoxyuridine (BrdU) immunostaining after culturing for 3 days. (A-L) Single labeling with (A-F) DAPI And (G-L) BrdU. (M-R) Double labeling with DAPI and BrdU; note the greater amount of nuclear staining in WT-derived cultures. (S) Graph showing the ratio of BrdU:DAPI; note that WT cell cultures displayed a greater proliferation rate, especially at 72 hours. (T) Graph showing the astrocyte number for each day; the absence of TLR2 affected the cell growth after 48 hours. ** P<0.01; *** P<0.001. Scale bar:50 Î¼m.